User:Wayne Decatur/Sandbox Thermus 70S With Paromomycin

Ribosome interactions with Paromomycin

Mechanism of action
NEED TO DO STILL: SSU alone with PAR

Ramakrishnan one with Par

The 70S is so large that the small paromomycin molecule is not readily obvious, and so here the view zooms in and paromomycin is highlighted by making the ribosome partially translucent.

Returning to the initial scene of the full ribosome now aware of the locale where paromomycinin is bound, it is clear that in the Thermus 70S paromomycin is observed binding to the penultimate helix of the small subunit (helix 44) at the decoding center where the anticodon stem-loop of the A-site tRNA contacts the mRNA (shown as yellow).

NEED TO DO STILL: Add the e. coli 70S that has two molecules bound

About these Structure
2j00 is a 25 chains structure of sequences from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA.

2j01 is a 31 chains structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA. Versions of 2j00 and 2j01 optimized for use online as described here are used on this page. You may wish to examine the ADD LINK basics of the subunit.

Related Structures

 * Haloarcula Large Ribosomal Subunit
 * Interactions between Antibiotics and the Ribosome

Additional Literature and Resources

 * Lecture by Venki Ramakrishnan in 2004 entitled The Ribosome: The Cell's Protein-Synthesizing Machine and How Antibiotics Disrupt It - posted on Brookhaven National Laboratory's Channel
 * Paramomycin at the DrugBank database
 * Paromomycin sulfate at Wikipedia